Weighted Gene Co-expression Network Analysis to Identify Key Modules and Hub Genes Related to Withanoloides Biosynthesis Pathway in Datura metel
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Date
2022-11-25
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Nsbm Green University
Abstract
The gene co-expression analysis and clustering concept were utilized to identify possible genes present in the withanolides biosynthesis pathway. Weighted Gene Co-expression Network Analysis (WCGNA) was carried out to identify putative genes. The identified known genes were used as bait to locate the co-expressed genes among them in identified modules in WGCNA analysis. As cytochrome P450s and glycosyltransferase (UGT) are candidates for withanolide biosynthesis in the literature, the study was focused on identifying CYPs and UGT genes. Finally, the study was able to locate 6 CYP genes and 1 UGT gene as putative candidate genes involved in the withanolides biosynthesis pathway. However, in silico characterization should be carried out for further isolation processes.
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Hewadikaram,M., De Silva, K. (2022) , Weighted Gene Co-expression Network Analysis to Identify Key Modules and Hub Genes Related to Withanoloides Biosynthesis Pathway in Datura metel International Conference On Business Innovation (ICOBI), NSBM Green University , Sri Lanka. P. 478-485